Background of F(ab')2

Background of F(ab')2

Comparing F(ab')2 fragment antibodies to entire IgG antibodies reveals both benefits and drawbacks. Positively, because they lack the Fc region, they do not result in non-specific binding or immune complex-mediated allergic responses. Furthermore, increased tissue penetration made possible by their lower molecular weight enhances the sensitivity and effectiveness of antigen recognition. Their divalent nature allows them to bind and precipitate antigens concurrently, making them appropriate for use in immunoprecipitation, immunoblotting, and other investigations. They are inappropriate for ELISA, flow cytometry, and other investigations, nevertheless, since they lack the Fc region, which prevents them from being recognized by complement or secondary antibodies. Because of their lower molecular weight, they have a short half-life, are readily eliminated by the kidneys, and necessitate higher dosages or more frequent administration.